牛磺酸对大鼠肺动脉平滑肌细胞凋亡的影响

Taurine induces apoptosis in pulmonary artery smooth muscle cells

目的:观察Tau对肺动脉平滑肌细胞(PASMCs)凋亡作用的影响,并探讨其机制是否通过死亡受体途径。方法:取Wistar雄性大鼠肺动脉平滑肌细胞培养;分为正常组(control)、凋亡组(SD)、给药组(Taurine高、中、低剂量)。用吖啶橙(AO)法,在荧光显微镜下观察6孔板细胞核形态学变化;用Western-blot法分别测定Bax,Bcl-2,Procaspase-3,Fas的蛋白表达变化。结果:吖啶橙法3组结果可以看出,凋亡组和Tau给药组的细胞核皱缩,形成凋亡小体。Western-blot法测定显示Tau升高Bax和Fas蛋白表达,降低Procaspase-3和Bcl-2的表达。结论:Tau可能通过死亡受体途径诱导肺动脉平滑肌细胞凋亡。

Objective： To study the effect of taurine on apoptosis in PASMCs,and whether the death-receptor pathway act in the mechanism.Method： Culture the PASMCs,and divided the cells into control,SD.Acridine orange（AO） assay and western-blot analysis on the expression of Bax,Bcl-2,Procaspase-3 and Fas were used to study the mechanism.Result： A major finding of this study is that the Tau effects many apoptosis index,such as increasing the expression of Bax and Fas,decreasing the expression of Procaspase-3,and Bcl-2,accrescencing the mitochondrial depolarization,causing the nuclear shrinkage,all these datas demonstrated that Tau induced the apoptosis in pulmonary artery smooth muscle cells through mitochondrial-dependent pathway.Conclusion： Tau induces the apoptosis in pulmonary artery smooth muscle cells through death-receptor.