胡椒酸丁二醇单脂与牛血清白蛋白相互作用的研究

Studies on the interaction between new anti-hyperlipidemic compounds and bovine serum albumin

运用荧光及紫外-可见吸收光谱法研究了胡椒酸丁二醇单酯(简称BPM)与牛血清白蛋白(BSA)的相互作用。实验结果表明,胡椒酸丁二醇单酯与BSA形成基态复合物导致BSA内源性荧光猝灭,猝灭机理主要为静态猝灭和非辐射能量转移,其猝灭速率常数为Kq为1.077×1013L/(mol.s)(25℃)、0.946×1013L/(mol.s)(37℃)。利用荧光猝灭反应测得结合常数KA为2.6×106(25℃)、3.4×106(37℃),结合位点数n为1.30(25℃)、1.33(37℃)。根据Frster能量转移理论得到结合距离r=2.92nm(25℃)、2.66nm(37℃)和能量转移效率E=0.45(25℃)、0.43(37℃)。通过热力学参数计算,确定胡椒酸丁二醇单酯与BSA的相互作用是熵增加和吉布斯自由能降低的自发过程,主要作用力是疏水作用力。

The interaction between butanediol piperinie mono-ester and bovine serum albumin （BSA） has been studied by fluorescence spectroscopy and UV-vis absorption spectroscopy. The experimental results showed that the fluorescence quenching of BSA by butanediol piperinie mono-ester was due to the formation of butanediol piperinie mono-ester-BSA complex through both static quenching and nonradiative energy transfer. The quenching constants （Kq） were 1. 077 × 10^13L/（ mol. s） （25 ℃ ） and 0. 946 × 10^13L/（ mol. s） （37 ℃ ）. With the fluorescence quenching method, the binding constants（ KA ） were 2.6 × 10^6L/moL（25℃ ）and 3.4 × 10^6L/moL （37 ℃ ）, and the values of binding sites （n） were 1.30 （25 ℃ ） and 1.33 （37℃）. According to the FSrster theory of non-radiation energy transfer, the b energy transfer efficiencies （ E ） were inding distance（r） were 2.92nm（25℃ ） and 2.66nm（37℃ ）, and the 0. 45 （ 25℃ ） and 0. 43 （ 37℃ ）. The binding process of binding was a spontaneous molecular interaction in which entropy increased and Gibbs free energy decreased, indicating that the hydrophobic force played major role in the interaction of butanediol piperinie mono-ester and BSA.