花菁-脱氧核糖核酸的共振光散射光谱及其分析应用

Resonance light scattering spectral study of the interaction of cyanine dye with nucleic acids and its analytical applications

研究了一种苯并噻唑阳离子花菁与脱氧核糖核酸(DNA)作用的共振光散射光谱,在pH 6.0的六次甲基四胺-HCl缓冲介质中,痕量DNA的加入使花菁在590nm的共振光散射强度显著增强。在最佳实验条件下,增强的共振光散射强度与DNA浓度具有良好的线性关系,据此建立了一种测定DNA的共振光散射光谱法。方法的线性范围为:小牛胸腺DNA(CT DNA),0～20μg/mL,鱼精子DNA(FS DNA),0～15μg/mL;检出限分别为0.005μg/mL和0.008μg/mL。该方法已用于合成样品中DNA的测定。

The resonance light scattering （RLS） spectra of benzothiazole cationic cyanine with deoxyribonucleic acid （DNA） have been studied. The RLS intensity of cyanine at 590nm is greatly enhanced by the addition of trace amounts of DNA in pH 6. 0 hexamethylene tetramine-HCl media. Under the optical experimental conditions, the enhanced RLS intensity was proportional to the concentration of DNA. The RLS method for the determination of DNA was established. The linear ranges of the method are 0 - 20μg/mL for CT DNA and 0 - 15 μg/mL for FS DNA, and the detection limits are 0. 005 μg/mL and 0. 008μg/mL respectively. The method has been applied to the determination of DNA in synthetic samples