固定化沙雷氏菌脂肪酶拆分(R,S)-2-羟基-4-苯基丁酸乙酯

Resolution of(R,S)-2-Hydroxy-4-ethyl benzene by Immobilized Serratia sp.Lipase

利用海藻酸钙-戊二醛交联法对本实验室筛选到的一株能选择性拆分(R,S)-2-羟基-4-苯基丁酸乙酯〔(R,S)-HPBE〕的沙雷氏菌脂肪酶进行固定化,并对固定化脂肪酶的酶活和拆分条件进行了研究。结果表明,最适反应温度为50℃,pH=7.0,底物浓度40 mmol/L,ρ(酶液)=200 g/L,在该条件下,反应10 h后,(R)-2-羟基-4-苯基丁酸乙酯〔(R)-HPBE〕产率达93.4%,光学纯度(e.e.)为96.2%。连续反应12批次,固定化脂肪酶仍可使(R)-HPBE产率维持在80%以上。

Optional resolution of （ R, S ） -2-hydroxy-4-ethyl benzene （ （ R, S ） -HPBE ） by calcium alginate-glutaraldehyde cross-linking Serratia sp. lipase was developed, and the activity of the immobilized lipase and many factors affecting the resolution were investigated. The optimum reaction conditions were ： temperature 50℃, pH = 7.0, concentration of （ R, S） -HPBE 40 mmol/L and p （ lipase amount） =200 g/L. After 10 h of resolution by immobilized lipase, the conversion rate of （R）-HPBE was 93.4% and e.e. was 96. 2%. After twelve times＇ repeated batch operation,the conversion rate of the product by immobilized lipase was more than 80%.