肝卵圆细胞与原发性肝癌的相关性研究

CORRELATION BETWEEN HEPATIC OVAL CELL AND PRIMARY HEPATIC CARCINOMA

目的:探讨肝卵圆细胞与原发性肝癌的相关性,为原发性肝癌发生的"癌干细胞"假说提供临床病理学依据。方法:运用免疫组化方法检测不同肝病组(检测对照组、肝血管瘤组、良性肝病合并肝硬化组、肝癌组、肝癌合并肝硬化组)肝脏组织中表达Ck-7、Ck-8/18、Ck-19、C-kit、AFP肝卵圆细胞数。结果:Ck-7、Ck-8/18、Ck-19阳性的肝卵圆细胞在上述各检测组中均有表达,AFP阳性的肝卵圆细胞仅在肝癌组中表达。各组间表达的Ck-7、Ck-8/18、Ck-19、C-kit、AFP阳性的肝卵圆细胞数目随病变由良性疾病向恶性疾病变化而增加。肝癌组、肝癌合并肝硬化组间表达的C-kit阳性的肝卵圆细胞数有显著性差异(P<0.05)。结论:肝卵圆细胞参与了原发性肝癌的生长、细胞分化调控。

Objective ：To investigate the correlation of hepatic oval cell with primary hepatic carcinoma for proving that the primary hepatic carcinoma is originated from hepatic oval cell. Methods：76 biopsy samples of liver tissue were taken from normal liver, hemangioma of liver, hepatic cirrhosis, primary hepatic carcinoma, and primary hepatic carcinoma complicated with cirrhosis; immunohistochemical method was used to measure thenumber of the cells which expressed cytokeratins-7（Ck-7）, cytokeratinsl9（Ck-19）,C-kit,cytokeratins-8/18（Ck-8/ 18）,and alpha-fetoprotein （AFP）. Results：,Hepatic oval cells in all the 5 groups could express Ck-7,Ck-8/18, C- kit and Ck-19.But AFP was only expressed in malignant groups. The number of hepatic oval cells expressed Ck- 7,Ck-8/18, C-kit,Ck-19,and AFP was increased and changed when benign disease became to malignant. Thenumber of C-kit positive hepatic oval cells was significantly different between primary hepatic carcinoma, and primary hepatic carcinoma complicated with cirrhosis. Conclusion：Hepatic oval ceils participate in primary hepatic carcinoma growth, and cell differentiation regulation.