泸州仙鹤草不同采收期总黄酮含量的测定

DETERMINATION OF TOTAL FLAVONOIDS OF AGRIMONIA PILOSA LEDEB.AT DIFFERENT HARVEST TIME IN LUZHOU WHICH LIVED

目的:对泸州地区不同采收期仙鹤草进行总黄酮的含量测定,建立其含量测定方法,为确定泸州地区仙鹤草的采收期提供依据。方法:采用紫外分光光度法测定不同采收期仙鹤草的总黄酮含量。结果:采用紫外分光光度法测定总黄酮,测得泸州仙鹤草中总黄酮在0.0055～0.088mg·ml^(-1)浓度范围内具有良好的线性关系(r=0.9997),平均回收率为98.15%,RSD为1.18%。泸州地区6月份采集的仙鹤草总黄酮含量为10.77%,7月份采集的仙鹤草总黄酮含量为12.38%,明显高于其它月份采集的仙鹤草中的总黄酮含量。结论:采用紫外分光光度法建立了泸州地区仙鹤草总黄酮含量测定的方法,该方法操作简便、快速、灵敏、精密度好、准确度高、具有良好的重复性和回收率。泸州地区仙鹤草采收期以6、7月份为宜。

harvest time, nation of total Objective ：To determine the content of total flavonoids of Agrimonia pilosa Ledeb. at different for selecting its best harvest time.Methods： Ultraviolet spectrophotometry was used for the determi- flavonoids of Agrimonia pilosa Ledeb. at different harvest time in Luzhou. Results： The content oftotal flavonoids could be detected by ultraviolet spectrophotometry. Total flavonoids had good linearity in the range of 0.0055~0.088mg.m1-1 （r=0.9997）, the average recovery was 98.15%, and RSD was 1.18%. The content of total flavonoids in Agrimonia pilosa Ledeb. in Luzhou was 10.77% in June, and 12.38% in July,much higher than in any other month. Conclusions： This experiment establishes a method using ultraviolet speetrophotometry to detectthe content of total flavonoids, it is simple, rapid, sensitive,with high precision, high accuracy and good repeata- bility and recovery. The reasonable harvest time of Agrimonia pilosa Ledeb.in Luzhou is June and July.