化痰通络方对大鼠胶原诱导性关节炎作用的实验研究

Effect of Huatan Tongluo Fang on collagen-induced arthritis in rats

目的观察化痰通络方对胶原诱导性关节炎(CIA)的治疗作用。方法选择牛Ⅱ型胶原为免疫原,建立CIA模型,选取40只造模成功的大鼠随机分为4组:生理盐水组(A组)、化痰通络方组(B组)、甲胺喋呤组(C组)、化痰通络方+甲胺喋呤组(D组),每组各10只,疗程30 d。采用关节炎指数评分(AI)对大鼠关节炎程度进行评分;采用酶联免疫吸附法(ELISA)测定各组大鼠血清基质金属蛋白酶-3(MMP-3)、血管内皮生长因子(VEGF),根据滑膜病理评估滑膜炎指数积分。结果 AI评分:A组治疗前后AI明显升高(P<0.01),C组与D组治疗前后AI明显降低(P<0.01);B组、C组、D组AI均低于A组(P<0.05,P<0.01,P<0.01),D组AI低于B组(P<0.05)。血清VEGF、MMP-3检测结果显示:B、C、D组均显著低于A组(P<0.01);D组显著低于B组(P<0.05),而B组与C组、C组与D组差异不显著(P>0.05)。滑膜炎指数积分(SMII)评估结果:B、C、D组SMII均低于A组(P<0.01);D组SMII低于B组(P<0.05);D组SMII低于C组,C组SMII低于B组,但差异不显著(P>0.05)。结论化痰通络方能减轻CIA关节炎症;化痰通络方能明显降低血清VEGF、MMP-3水平;化痰通络方与甲胺喋呤(MTX)联合使用可进一步增强疗效。

Objective To observe the curative effect of Huatan Tongluo Fang on collagen-induced arthritis （CIA） in rats. Methods The CIA model was established through choosing bovine collagen II as immunogen, and then modeled rats （n = 40） were randomly divided into normal saline group （group A）, Huatan Tongluo Fang group （ group B） , methotrexate group （ group C ） and Huatan Tongluo Fang ＋ MTX group （group D, each n = 10）. A therapeutic course was 30 days. The degree of CIA was scored by using arthritis index （AI） scoring. The levels of serum matrix metalloproteinase-3 （ MMP-3 ） and vascular endothelial growth factor （VEGF） were detected by using enzyme-linked immunosorbent assay （ELISA）. The integrals of synovitis index were reviewed based on synovial pathology. Results The scores of AI increased significantly in group A（P 〈0.01 ）, had no changes in group B（P 〉0.05） and decreased significantly in group C and D（P 〈0. 01 ）. The scores of AI was significantly lower in group B, C and D than those in group A （ P 〈 0.05 ）, and significantly lower in group D than in group B （P 〈 0.05）. The levels of VEGF and MMP-3 were lower in group B, C and D than those in group A （P〈O. 01）, and lower significantly in group D than in group B （P〈0. 05）. The difference between group B and C, or between group C and D was not significant （P 〉 0.05）. The integrals of synovitis index were lower significantly in group B, C and D than those in group A, and lower in group D than ingroup B （P 〈 0.05 ）, lower in group D than in group C, and lower in group C than in group B but the difference was not significant （P 〉 0. 05 ）. Conclusion Huatan Tongluo Fang can relieve CIAinflammation and reduce signiticanfly the levels of serum VEGF and MMP-3. Its curative effect will be enhanced when it combined with MTX.