糖尿病大鼠膀胱逼尿肌超微结构的改变

Ultrastructural changes of bladder detrusor of non-insulin dependent diabetes mellitus rats

目的观察2型糖尿病大鼠膀胱逼尿肌超微改变,从细胞形态学分析糖尿病膀胱功能障碍发病机理。方法建立STZ诱导糖尿病大鼠模型,在4、12周时电镜下观察膀胱逼尿肌超微结构。结果电镜下逼尿肌表现为逼尿肌细胞体积增大,SMC之间的间隙缩小,细胞间胶原纤维增多,细胞间中间连接减少,胞突连接及缝隙连接多见。12周时上述改变较4周明显加重。与时间有明显相关性,此损害过程为一个神经纤维束减少、膀胱逼尿肌代偿与重建,最后导致膀胱功能障碍的过程。结论糖尿病早期,大鼠膀胱逼尿肌的平滑肌、细胞间质和神经纤维均有改变,随着时间的延长变明显。形态学方面的检测为神经电生理检查和尿动力学提供佐证,为临床诊断治疗糖尿病膀胱病提供依据。

Objective To investigate pathological changes of the diabetic rats detrusor and pathogenesis of bladder dysfunction. Methods STZ induction diabetes rat model was established,the ultrastructure of detrusor of rats in control group and observation group was observed 4 and 12 weeks latert under electron microscope. Results The detrusor under the electron microscope demonstrated the increase of detrusor muscle cell volume, the gap between SMC was narrowing, collagen fibers among cells increased, intercellular connections reduced,bursting cellular connection joints and connections. Such changes were significant at 12 weeks than at 4 weeks,and apparenhy associated with time the damage resulted in a process of a nerve fiber bundle reduction, adaptation and reconstruction of bladder detrusor muscle, and finally dysfunction of the urinary bladder. Conclusion The smooth muscle, interstitial cells and nerve fibers of diabetic rat bladder detrusor, have changed in the early stage under the electron microscope,with the extension of the time,this change significantly,may affect bladder function. Detection of morphology can accurately provide support for urodynamic and nerve electrophysiological examination, accurate and provide the basis for chnical diagnosis and treatment of diabetic bladder disease.