表面活性剂对脂肪酶活性和选择性的影响

Effect of Surfactants on lipase Activity and Enantioselectivity

考察了几种表面活性剂对lipase OF粗酶和纯化酶催化拆分酮基布洛芬的影响.除吐温-80,吐温-60和壬基酚聚氧乙烯醚外,大部分表面活性剂对酶活性有抑制作用,其中只有吐温-80能显著提高酶的立体选择性.酶的活性和选择性与表面活性剂浓度有关.在表面活性剂浓度为最佳(20mg/mL吐温-80或30mg/mL壬基酚聚氧乙烯醚)时lipase OF粗酶的活性可分别提高13和15倍.加入80mg/mL吐温-80,粗酶和纯化酶的对映体选择率(E值)分别由1.1和8/0增至6.7和>100.

The enantioselective hydrolysis of 2 - chloroethyl ester of ketoprofen [ 2 - (3 - benzoylphenyl) propionic acid] was carried out by using either the crude or purified lipase from Candida rugosa (lipase OF) as biocatalyst. The effect of various surfactants on the reaction was investigated. The enzyme activity was found to increase significantly upon the addition of Tween - 80, nonyl phenol polyethyleneoxy ether or Tween - 60, while most of the other tested surfactants had inhibitory effects on the lipase. As for the enantioselectiviry, only Tween - 80 displayed a positive effect. The activity and the enantioselectivity of the enzyme were found to be sensitive to the concentration of Tween - 80. The activity was enhanced up to 13 and 15 times respectively in the presence of Tween - 80 (20mg/mL) or nonyl phenol polyethyleneoxy ether (30mg/mL) . In the case of 80mg/mL of Tween - 80, the enantiomeric ratios (E values) were increased from 1.2 to 6.7 and from 8 to > 100 respectively for the crude and purified lipase OF.