高分辨率熔解曲线分析在耐药菌检测中的应用研究

Application of high resolution melted curve analysis in detection of antibiotic-resistant bacteria

目的高分辨率熔解(High Resolution Melting,HRM)曲线分析是近几年来在国际上刚兴起的单核苷酸多态性及突变研究工具。这种检测方法因其高通量、低成本、结果准确、不受检测位点的局限,并且实现了真正的闭管操作而受到普遍的关注。现对HRM技术的原理和在耐药菌检测中的应用进展作一综述。

Objective High resolution melting （HRM）curve analysis is a fast and post-PCR high-throughput method for scanning the sequence variations in a target gene,which has just appeared in the past few years. HRM has several advantages over other genotyping and scanning methods,including an inexpensive closed tube format that is homogenous,accurate and rapid. The principle and application of HRM in molecular diagnosis as well as the research progress on HRM are demonstrated.