温度对酶抑制法测定蔬菜有机磷和氨基甲酸酯类农药残留量的影响

Study on the influence of temperature on determination of organophosphorus and carbamate pesticide residues in vegetable by enzyme inhibition methods

以抑制率为评价指标,采用酶抑制法快速测定大白菜和韭菜样品加农药标准品后抑制率的变化情况,研究不同培养温度和测定温度对大白菜和韭菜中甲胺磷和克百威残留量的影响。试验结果表明:不同培养温度和测定温度均会影响乙酰胆碱脂酶的活性,25℃和37℃的培养温度和测定温度对酶的活性影响较小,实际分析过程可选用25℃作为蔬菜中有机磷和氨基甲酸酯类农药残留量的快速培养温度和测定温度。

The rate of inhibition as the evaluation and the enzyme inhibition method was used to determine the change of pesticide residues rapidly of the cabbage and leek samples added the standards of pesticide.The paper also studied the effect of the different temperatures of culture and determine to the residue of the methamidophos and carbofuran in cabbage and chives.The results showed that: the activity of acetylcholinesterase was affected by the temperature of culture and measurement and the affection of 25℃ and 37℃ could be neglected.We can use the outcome of 25℃ as the culture and measure temperature of the determination of pesticide residues in the actual analysis process of phosphorus and organic in vegetables.