小鼠便秘模型的建立

Development of constipation model of mice

为研究保健食品的润肠通便功能，针对保健食品润肠通便功能试验的要求，用１０ｍｇ／ｋｇ ＢＷ的复方地芬诺酯建立了稳定的小鼠便秘模型，模型组与对照组在首粒黑便排便时间、６ ｈ内排便粒数及排便重量间均有显著性差异（Ｐ＜ ０ ．０５） 。多次重复实验表明，该模型稳定可靠，建议在保健食品润肠通便功能检测中对其进行验证。

Diphenoxylate was administrated to mice by the dose of 10 mg/kg BW for the development of constipation model for formulation of examination procedure of health foods. The time of defecation of the first black urine, quantity and weight of urine with 6 hours were statistically significant different compared with the control group. The results presented the stability and reliability of the model and it was recommended to verify in the function experiments of health foods.