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木霉拮抗烟草赤星病菌菌株的筛选及拮抗机制 被引量:9

Screening for the Isolates of Thrichoderma Viride Against Alternaria Alternata and Its Antagonistic Mechanism
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摘要 利用诱捕法从土壤中分离得到 18株木霉 (Trichodermasps.)菌株 ,采用对峙法从中测定、筛选出一株对烟草赤星病菌 (Alternariaalternata)具有极强拮抗作用的菌株 (Tv - 1)。光学显微镜及透射电镜观察结果表明 ,Tv - 1菌株的拮抗机制主要为生长竞争、寄生作用以及产生抗菌素类物质使病菌菌丝消解 ,表现为Tv - 1菌株菌丝生长迅速 ;能附着、缠绕于病菌菌丝上 ,并产生吸器侵入菌丝 ;同时还能分泌使病菌菌丝原生质浓缩、断裂。 By the method of baiting, 18 isolates were obtained ,and further identificatied as Trichoderma viride. The antagonistic tests of all these isolates to 7 strains of Alternaria alternata from diseased tobacco leaves from different area of Yunnan were carried out by pairing culture on PDA. The results showed that 1 isolated (Tv 1) was strong antagonistic. The examination of antagonistic mechanism under optical microscope and transmission electron microscope showed Tv 1 hyphae growed quickly. Its hyphae could adhere to, coil around the hyphae of pathogen. Its finger shaped haustorium penetrated into the hyphae of Alternaria alternat. Tv 1 could also secret antibiotic substance to make the pathogan hyphal plasma contracted, vacuolated and disrupted.
出处 《烟草科技》 EI CAS 2000年第3期45-47,共3页 Tobacco Science & Technology
关键词 赤星病菌 木霉 筛选 拮抗菌 拮抗机制 烟草 Alternaria alternata Trichoderma virid Screening Antagonistic organism Atagonistic mechanism
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  • 1李良,生物防治通报,1985年,1卷,1期,19页
  • 2[德]唐姆茨(K·H·Domsch),[荷兰]盖姆斯(W·Games) 著,韩绍英.农业土壤真菌[M]科学出版社,1979.

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