雷公藤多苷对胶原诱导性关节炎大鼠表皮生长因子受体家族表达的影响

Regulatory effect of tripterygium wilfordii polyglycoside on expression of epidermal growth factor receptor family in collagen induced arthritis rats

目的观察表皮生长因子受体（EGFR）家族成员EGFR、ErbB-2在胶原诱导性关节炎（CIA）大鼠滑膜及软骨中的表达及雷公藤多苷（TWP）对其影响，探讨EGFR、ErbB．2在CIA大鼠发病中的作用及TWP治疗RA的药理作用机制。方法建立CIA大鼠模型，分别采用免疫组织化学染色及实时定量聚合酶链反应（PCR）检测EGFR、ErbB-2在滑膜和软骨中的表达。统计学处理采用单因素方差分析。结果EGFR、ErbB-2的蛋白表达及mRNA水平在CIA模型组滑膜（EGFR0．268±0．059，ErbB-20．25±0．04，EGFRmRNA：14．2±0．55，ErhB-2mRNA23．46±3．64）和软骨（EGFR0．193±0．018，ErbB．20．217±0．033，EGFRmRNA：4．16±0．50，ErbB-2mRNA9．23±0．66）中明显高于正常组（P〈0．01），在治疗组中表达低于模型组（P〈0．01）。结论初步证明EGFR、ErhB-2参与了CIA的病理过程，邢P治疗RA的机制可能与其降低EGFR、ErbB-2的表达有关。

Objective To study the regulatory effect of Tripterygium wilfprdii polyglycoside （TWP） on the expression of EGFR and ErbB-2 induced arthritis rats. The effect of TWP on arthritis was also explored. Methods After the model of CIA rats were established, the expression of EGFR and ErbB-2 in the synovium and articular cartilage were tested by immunohistochemical stain and real time PCR. ANOVA was used for statistical analysis. Results The protein and mRNA expression of EGFR and ErbB-2 in the synovium （EGFR 0.268±0.059,ErbB-2 0.25±0.04,EGFR mRNA： 14.2±0.55,ErbB-2 mRNA 23.46±3.64） and articular cartilage （EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA：4.16±0.50,ErbB-2 mRNA 9.23±0.66） of the model group were significantly higher than those of the control group （P〈0.01）. After being treated with TWP and MTX, the protein and mRNA expression of the EGFR and ErbB-2 decreased markedly （P〈0.01）. Conclusion EGFR and ErbB-2 may play an important role in the pathogenesis of arthritis development. The molecular mechanism that TWP can treat synovitis and bone destruction of RA is related to the inhibition of EGFR and ErbB-2.