成都地区汉族群体C_3表型频率调查与血痕中C_3表型的检测

INVESTIGATION ON THE C_3 PHENOTYPE FREQUENCIES IN THE HAN POPULATION IN CHENGDU AREA AND C_3 PHENOTYPING IN HUMAN BLOOD STAINS USING CELLULOSE ACETATE ELECTROPHORESIS FOLLOWED BY IMMUNOFIXATION

用醋酸纤维素薄膜电泳免疫固定法,调查了成都地区汉族群体 C_3表型频率的分布。在400例无关健康献血员中发现三种 C_3表型,SS 型397例,FS 型2例,SS_(var)型1例,其基因频率是 C_3~S=0.9963,C_3~F=0.0025,C_3^(Svar)=0.0013。表现型观察值与期望值吻合(p>0.95)。37℃和室温中放置2天的血痕,4℃中放置23天的血纱布和放置35天的玻璃上血痕,-20℃中放置至少87天血液的 C_3表型,可全部检出。人血清在室温中放3天,4℃中放13天,-20℃中至少放106天,可以全部检出 C_3表型。5例亲子鉴定案中检查了 C_3系统。

The distribution of C_3 phenotype frequencies in the Han population in Cheng-du area was studied by means of cellulose acetate electrophoresis followed byimmunofixation.In 400 unrelated healthy individuals three C_3 phenotypes weredemonstrated.Their frequencies were as follows:SS=397,FS=2 and SSvar=1.Their gene frequencies were as follows:C_3~S=0.9963,C_3~F=0.0025 and C_3^(svar)=0.0013.The C_3 phenotype frequencies were in good agreement with those expec-ted.C_3 phenotyping in the huma bloodstains kept at 37℃ and room temp-lasserature for two days,at 4℃ for 23 days(cotton bloodstains)and for 35 days(g-bloodstains),and -20℃ for at least 87 days could be performed.C_3 ph-enotypingin human serum could be performed at roon temperature for 3 days,as 4℃ for 13days and at -20℃ for at least 106 days.C_3 phenotyping was usedin five casesof paternity dispute.