高原红细胞增多症大鼠模型的建立

ESTABLISHMENT OF A RAT MODEL OF HIGH ALTITUDE POLYCYTHEMIA

目的建立一种简便、有效、可行的高原红细胞增多症大鼠模型,用于筛选和评价高原红细胞增多症防治药物。方法 16只雄性SD大鼠随机分为常氧组和低氧组,每组8只。低氧组大鼠每天在自制常压低氧舱(向舱内充9.8%氧含量的氮气和空气的混合气,模拟海拔高度6000 m)中停留8 h。分别在低氧处理后20 d、30 d、40 d、50 d、60 d和70 d时用氰化高铁血红蛋白法检测血红蛋白,以血红蛋白含量>210 g/L为模型建立成功的标准。结果低氧组大鼠在低氧处理40 d时血红蛋白含量为(234.1±10.7)g/L,40 d后血红蛋白含量维持在约230 g/L的较高水平。结论成功建立了简便、有效、可行的高原红细胞增多症大鼠模型,可用于高原红细胞增多症防治药物的筛选和评价。

Objective To establish a simple and effective high altitude polycythemia （HAPC） animal model for evaluating new drugs for treatment of HAPC. Methods Sixteen male SD rats were randomly divided into hypoxia group and normoxia group, with 8 rats in each group. The rats assigned to the hypoxia group were exposed to hypoxia for 8 hours per day in an atmospheric hypoxia chamber （injecting a 9.8 % 02 tension mixture of nitrogen and air into the chamber to simulate 6000 m high environment）. The Hb content of rats was detected by the hemoglobincyanide method on the 20th, 30th,40th,50th,60th and 70th day. And 210 g/L of Hb content was used as the criterion to judge the rat polycythemia. Results The Hb content of rats in the hypoxia group was （ 234.14 ± 10.66 ） g/L on the 40th day and lasted at about 230 g/L for 1 month. Conclusion A simple and effective HAPC rat model was established successfully. This model showed some advantages for drug evaluation.