沙雷氏菌发酵蓝藻生产蛋白酶

Protease production by Serratia sp. SYBC H with blue algae

以富含蛋白质的蓝藻作有机氮源进行微生物发酵生产蛋白酶,为蓝藻的资源化利用提供一条新途径。采用单因素实验,研究了影响沙雷氏菌发酵蓝藻生产蛋白酶的培养基主要成分。结果发现,在实验范围内,沙雷氏菌发酵蓝藻生产蛋白酶的最佳碳源是蔗糖,最佳速效氮源是尿素,最佳金属离子(盐)是ZnSO4,最佳产酶促进剂是吐温80。在单因素实验中,发酵上清液的蛋白酶活最高可达到941 U/mL,而且发酵周期短,发酵培养18 h即可达到最高酶(活)值。

The microbial fermentation of protein-rich blue algae for proteases production provides a new way to utilize the blue algae resource.The main factors of the fermentation medium affecting proteases production were studied by Serratia sp.SYBC H via the single factor experiment.The results showed that within the experimental ranges,the optimal carbon source,inorganic nitrogen source,salt and stimulant for Serratia sp.SYBC H proteases production were sucrose,urea,ZnSO4 and Tween 80,respectively.The highest protease activity reached 941 U/mL in the supernatant.The fermentation time is short.Only 18 h is required to reach the maximum protease activity in the supernatant.