和厚朴酚对小鼠黑色素瘤B16细胞增殖以及黑色素合成的影响

Inhibition of honokiol on the proliferation and melanin biosynthesis on B16 melanoma cells in vitro

目的探讨和厚朴酚对小鼠黑色素瘤B16细胞增殖以及细胞内黑色素合成的影响。方法体外培养小鼠B16细胞,MTT法检测和厚朴酚对B16细胞增殖的影响;DAPI染色法观察和厚朴酚对B16细胞细胞形态的影响;NaOH裂解法检测和厚朴酚对黑色素含量的影响。结果和厚朴酚浓度为51、0、204、0、80μmol/L作用B16细胞,在不同的用药时间122、4和48 h,和厚朴酚对B16细胞增殖的IC50分别为23.41、3.1和11.4μmol/L;同时和厚朴酚作用B16细胞12 h后,经DAPI染色,B16细胞呈现典型的凋亡形态;另外采用204、0、80μmol/L的和厚朴酚分别作用B16细胞,B16细胞内黑色素合成量也呈现降低的趋势。结论和厚朴酚能有效地抑制B16细胞增殖,且其抑制作用具有时间和浓度依赖性;药物作用后的B16细胞呈现凋亡形态并出现凋亡小体;和厚朴酚对B16细胞内黑色素合成也呈现抑制作用但不明显。

Objective To investigate the inhibition of honokiol on proliferation and melanin biosynthesis in B16 melanoma cells in vitro.Methods B16 cells were cultured in vitro.MTT assay,DAPI and NaOH lysis test were applied for assessment of cell proliferation,cellular morphologic observation and cellular melanin content,respectively.Results B16 cells were treated with honokiol with different concentrations（5,10,20,40 and 80 μmol/L） and durations（12,24 and 48 h）.The IC50 of honokiol on proliferation of B16 cells were 23.4,13.1 and 11.4 μmol/L,respectively,with durations of 12,24 and 48 h.Apoptosis of B16 cells were induced with honokiol in DAPI staining.Furthermore,the melanin biosynthesis was inhibited with honokiol with concentration over 20 μmol/L.Conclusion Honokiol effectively inhibits the proliferation of B16 in dose-and time-depend manners.Apoptotic bodies were observed in B16 cells treated with honokiol.Although melanin biosynthesis is also inhibited by honokiol,this effect is insignificant.