维药药西瓜化学成分的分离与鉴定

Isolation and identification of chemical constituents from Citrullus colocynthis

目的研究药西瓜Citrullus colocynthis的化学成分。方法采用硅胶、Sephadex LH-20等色谱手段结合重结晶技术进行分离纯化,通过NMR波谱数据和理化性质确定化合物的结构。结果从药西瓜中分离得到11个化合物,分别鉴定为β-谷甾醇(1)、α-菠甾醇-3-O-β-D-吡喃葡萄糖苷(2)、α-菠甾酮(3)、双-[(2-乙基)己基]邻苯二甲酸酯(4)、对羟基苯甲酸(5)、6-C-对甲基苄基牡荆素(6)、双氢葫芦素E(7)、葫芦素E(8)、异表双氢葫芦素D(9)、双氢异葫芦素B-25-乙酯(10)、葫芦素E-2-O-β-D-吡喃葡萄糖苷(11)。结论化合物6为新化合物,命名为6-C-对甲基苄基牡荆素;化合物1～5、7、9、10均为首次从该属植物中分离得到。

Objective To study the chemical constituents from Citrullus colocynthis.Methods The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography together with recrystallization methods and their structures were identified by NMR spectra and physicochemical property.Results Eleven compounds were isolated and identified as β-sitosterol（1）,α-spinasterol-3-O-β-D-glucopyranoside（2）,α-spinasterone（3）,bis（2-ethylhexyl） phthalate（4）,p-hydroxybenzoic acid（5）,6-C-p-methylbenzylvitexin（6）,dihydrocucurbitacin E（7）,Cucurbitacin E（8）,dihydro-epi-iso-cucurbitacin D（9）,dihydroisocucurbitacin B-25-acetate（10）,and cucurbitacin E-2-O-β-D-glucopyranoside（11）.Conclusion Compound 6 is a novel compound.Compounds 1―5,7,9,and 10 are isolated from the plants in Citrullus Schrad.ex Eckl.et Zeyh.for the first time.