金丝李茎皮化学成分研究

Chemical constituents from stem barks of Garcinia paucinervis

目的对金丝李Garcinia paucinervis的茎皮化学成分进行分离鉴定,并进行抗肿瘤活性筛选。方法采用硅胶、反相RP-18,制备薄层色谱,Sephadex LH-20等柱色谱方法对乙醇提取物石油醚萃取部位化学成分进行分离和纯化,并根据其理化性质及波谱数据鉴定结构;采用MTT法进行抗肿瘤活性筛选。结果从金丝李乙醇提取物中分离得到10个化合物,分别鉴定为cambogin(1)、焦袂康酸(2)、β-谷甾醇(3)、胡萝卜苷(4)、7-prenyljacareubin(5)、parvifolixanthone A(6)、formoxanthone A(7)、termicalcicolanone A(8)、1,3,5,6-tetrahydroxy-4-prenylxanthone(9)、isogarcinol(10)。抗肿瘤活性筛选结果表明,化合物5和7对HL-60、SMMC-7721、A549、MCF-7和SW480细胞株有一定的抑制作用。结论化合物2～5和7～10均为首次从该植物中分离得到,同时首次报道了化合物5的13C-NMR数据。

Objective To study the chemical constituents from the stem barks of Garcinia paucinervis and their antitumor activities.Methods Chemical constituents were isolated and purified from petroleum ether extract of G.paucinervis by chromatography on silica gel,RP-18,Sephadex LH-20 column,and preparative TLC.Their structures were identified on the basis of spectroscopic analysis and chemical evidence.The antitumor activity was screened by MTT assay.Results Ten compounds were isolated and identified as cambogin（1）,pyromeconic acid（2）,β-sitosterol（3）,daucosterol（4）,7-prenyljacareubin（5）,parvifolixanthone A（6）,formoxanthone A（7）,termicalcicolanone A（8）,1,3,5,6-tetrahydroxy-4-prenylxanthone（9）,and isogarcinol（10）.Compounds 5 and 7 showed moderate cytotoxic activity against HL-60,SMMC-7721,A549,MCF-7,and SW480 cell lines.Conclusion Compounds 2—5 and 7—10 are isolated from this plant for the first time and the13C-NMR spectroscopic data of compound 5 are firstly reported.