蝎毒多肽提取物对人卵巢癌SKOV3细胞增殖及血管内皮生长因子和凝血酶敏感蛋白表达的影响

Effects of polypeptide extract from scorpion venom on cell proliferation and protein expression of VEGF and TSP-1 in human ovarian cancer cell line SKOV3

目的观察蝎毒多肽提取物(简称蝎毒多肽)对人卵巢癌SKOV3细胞增殖及血管内皮生长因子(VEGF)、凝血酶敏感蛋白(TSP-1)表达的影响,进一步探讨蝎毒多肽的抗肿瘤作用机制。方法 MTT法检测蝎毒多肽对SKOV3细胞增殖的影响;免疫细胞化学法及Western blotting测定蝎毒多肽对SKOV3细胞VEGF、TSP-1蛋白表达的影响。结果蝎毒多肽12.5～200μg/mL对SKOV3细胞增殖有抑制作用,且呈明显的质量浓度相关性,质量浓度大于50μg/mL时细胞增殖抑制作用显著(P<0.01)。随着蝎毒多肽质量浓度的增加,SKOV3细胞TSP-1蛋白表达增加,VEGF蛋白表达下降(P<0.01)。结论蝎毒多肽能显著抑制VEGF的表达,促进TSP-1的表达,其抗肿瘤作用机制可能是通过抑制肿瘤细胞增殖、影响肿瘤细胞血管生成过程中关键因子的表达来实现的。

Objective To observe the effects of polypeptide extract from scorpion venom（PESV） on the cell proliferation and protein expression of vascular endothelial growth factor（VEGF） and thrombospondin-1（TSP-1） in human ovarian cancer cell line SKOV3 and to investigate the possible mechanism of its antitumor effect.Methods Ovarian cancer cell line SKOV3 was cultured by the routine method,after being treated with PESV at different concentrations,the inhibition of cell proliferation was tested by MTT assay;Immunocytochemistry and Western blotting were applied to detecting the effects of PESV on protein expression of VEGF and TSP-1.Results In a dose-dependent manner,PESV remarkably inhibited the proliferation of SKOV3 at the range of 12.5—200 μg/mL.PESV（ 50 μg/mL） had a significant inhibitory effect on cell proliferation（P0.01）.Western blotting and immunocytochemistry results showed that with the increasing of PESV concentration,the expression levels of VEGF decreased while TSP-1 increased gradually（P0.01）.Conclusion PESV could significantly inhibit the expression of VEGF,but improve the expression of TSP-1.Antitumor effects of PESV may be carried out by inhibiting the tumor cell proliferation and impacting the expression of key factors in the process of tumor angiogenesis.