刺松藻水溶性多糖抗肿瘤及免疫调节作用研究

Antitumor activity of water-soluble polysaccharides from Codium fragile and its immunomodulation

目的研究刺松藻水溶性多糖(WSPCF)抗肺癌和免疫调节作用。方法采用MTT法检测WSPCF对人肺腺癌A549细胞体外生长的抑制作用,对正常C57BL/6J小鼠脾淋巴细胞增殖的影响和对脾淋巴细胞与巨噬细胞抑瘤作用的影响;中性红比色法检测WSPCF对C57BL/6J小鼠脾巨噬细胞吞噬活性的影响;检测WSPCF对Lewis肺癌荷瘤小鼠肿瘤生长、脾脏和胸腺的影响;采用ELISA法检测荷瘤小鼠的细胞免疫因子指标。结果 WSPCF质量浓度高于1 000μg/mL时,对A549细胞生长有抑制作用,500μg/mL时能显著增强淋巴细胞增殖和巨噬细胞吞噬活性(P<0.05),提高两者的抑瘤效果;与对照组相比,WSPCF对荷瘤小鼠的抑瘤率分别为39%、42%,差异有统计学意义(P<0.05),荷瘤小鼠血清中的免疫因子IL-1β、IL-2、IL-6、IL-10、IL-12、IL-18、IFN-γ、FAS-L水平均显著提高(P<0.05)。结论 WSPCF在体外对人肺腺癌A549细胞有一定的抑制作用,能提高小鼠细胞和分子免疫应答水平;在体内可改善Lewis肺癌荷瘤小鼠的免疫功能,通过激活机体免疫系统实现有效的抗肺癌活性和免疫调节作用。

Objective To investigate the anti-lung cancer activity and immunomodulation of water-soluble polysaccharides from Codium fragile（WSPCF）.Methods Inhibitory rate of WSPCF on A549 cell line,the C57BL/6J mice spleen lymphocyte proliferation,antitumor effect of spleen macrophages and lymphocytes were assessed by MTT assay.The effect of WSPCF on phagocytosis of C57BL/6J mice spleen macrophages was studied by neutral red colormetric method.The effect of WSPCF on the level of cytokine secretion,tumor growth,and the spleen and thymus of the Lewis lung cancer-bearing mice was detected by ELISA method and statistical analysis.Results In vitro,WSPCF,when the concentration was higher than 1 000 μg/mL,could inhibit the growth of A549 cells,while 500 μg/mL,it could significantly enhance the lymphocyte proliferation and phagocyte activity of macrophages（P0.05）,so as to improve their anti-tumor effects.In vivo,tumor inhibitory rates in WSPCF group were 39% and 42%,compared with the control group,the difference being statistically significant（P0.05）,the levels of IL-1β,IL-2,IL-6,IL-10,IL-12,IL-18,IFN-γ,and FAS-L in tumor-bearing mice serum,compared with the control group,were obviously improved（P0.05）.Conclusion WSPCF,has an in vitro inhibitory effect on human lung cancer A549 cells and could improve the level of cellular and molecular immune response.Also it could significantly improve the immune function of Lewis lung cancer-bearing mice and achieve the effective anti-lung cancer activity and immunoregulation through activating the immune system of the organism.