白玉菇液体发酵菌丝体生长和产胞外多糖培养基的优化

Mycelial growth of fermentation medium of white Hypsizigus marmoreus and the optimization of exopolysaccharides medium

白玉菇液体发酵培养基的最优配方可为白玉菇工业扩大培养提供基础资料。以葡萄糖、酵母粉、KH2PO4和MgSO4为主要因素,选择胞外多糖含量和菌丝体生物量为试验指标,采用L9(34)正交试验进行液体发酵培养基的优化。通过抽滤分离菌丝体,烘干称重,计算菌丝体生物量;用苯酚-硫酸法测定胞外多糖含量。试验测得通过对试验结果进行综合加权评分,确定了白玉菇液体培养基的最优配方为葡萄糖3%、酵母粉0.15%、KH2PO40.30%、MgSO40.20%。

The optimum liquid medium of white Hypsizigus marmoreus can provide the fundamental data for its extensive cultivation. We optimized fermentation medium with L9 （34） orthogonal test, the main ingredients of glucose, yeast powder, KH2PO4 and MgSO4, and exopolysaccharides content and mycelial biomass as test indexes. We also separated mycelia by extraction and filtering, dried and weighted them, and calculated their biomass. Phenol-sulfuric acid was then employed to determine the content of exopolysaccharides. Weighted evaluation of exopolysaccharides content and mycelial biomass shows that the optimum liquid medium is composed of glucose of 3%, yeast powder of 0. 15%, KH2PO4 of 0.30% and MgSO4 of 0.20%.