摘要
以罗非鱼Tilapia mossambica鱼鳞为原料,经低温酶解提取鱼鳞Ⅰ型胶原,将鱼鳞Ⅰ型胶原与壳聚糖进行混合交联,冻干后制成胶原-壳聚糖止血海绵。以止血海绵的密度、吸水倍数、保水率、透气率和止血时间为指标,通过单因素实验和正交试验优化获得最佳的制备工艺:胶原与壳聚糖配比7∶3、制备液总浓度1.4%、交联剂添加量0.015%。在该最佳工艺下制备的止血海绵密度为22.95mg/cm3、吸水倍数为45.34、保水率为55.83%、透气率为46.36%、止血时间为68.0s,呈蜂窝状多孔结构,具有优良的持水性和透气性。
Fish scale type I collagen was extracted through low temperature enzymolysis from TilaDia mossambica, and after being crosslinked with chitosan, hemostatic sponge wasgenerated via freeze-drying. Through single factor comparative tests and orthogonal tests, the optimum preparation technique was obtained as follows: mixing of collagen with chitosan according to a given volume ratio 7 : 3, maintaining total concentration of preparation mixture at1.4%, and keeping dosage of cross-linking agent at 0. 015%. Under this condition, the generated hemostatic sponge possessed excellent properties, such as density (22.95 mg/cm^3), waterabsorption (45.34), water retention (55.83%), breathability (46.36%), and hemostatic time (68.0 s). A faveolate vesicular structure, which contributed to preferable properties, was clearly observed with SEM.
出处
《渔业科学进展》
CSCD
北大核心
2012年第1期129-135,共7页
Progress in Fishery Sciences
基金
福建省自然科学基金(2008J0251)
福建省水产研究所科研发展基金资助项目共同资助
关键词
鱼鳞胶原
壳聚糖
止血海绵
交联
Fish scale collagen
Chitosan
Hemostatic sponge
Crosslinking