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胶质瘤U251细胞中CD133表达及干细胞特性分析 被引量：1

The detection and analysis of biological characteristics in CD133 positive cells of U251 glioma
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摘要 目的:探讨CD133+细胞的生物学特性,分析CD133作为胶质瘤干细胞标记的可行性。方法:利用免疫荧光染色检测U251细胞中CD133、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经胶质细胞纤维酸性蛋白(GFAP)等蛋白表达,分析CD133+细胞与CD133-细胞中巢蛋白、NSE、GFAP阳性细胞存在的差异。结果:U251中CD133+细胞比例为0.060±0.003,在CD133+细胞中,巢蛋白阳性细胞,NSE和GFAP阴性细胞比CD133-中明显增多;在CD133-细胞中,也存在巢蛋白阳性细胞,数目较CD133+中明显减少,相反巢蛋白阴性细胞,NSE和GFAP阳性细胞明显增多。结论:胶质瘤U251细胞存在CD133+细胞,在CD133阳性和阴性细胞中,均存在干细胞样细胞,但是在CD133+细胞中此类细胞更多。 Objective： To explore the biological characteristics of CD133 ＋ cells and analyze the feasibility of CD133 as the marker in glioma stem cells.Methods： The expression of CD133,nestin,neuron-specific enolase（NSE）,glial fibrillary acidic protein（GFAP） were detected by immunofluorescence in U251 glioma cells.The differences of the nestin,NSE,GFAP-positive cells were analyzed between CD133 ＋ cells and CD133-cells.Results： In the U251 cells,the CD133＋ cells composed to 0.060±0.003.In CD133＋ cells,nestin-positive cells,NSE and GFAP-negative cells increased significantly;in CD133-cells,on the contrary,nestin negative cells,NSE and GFAP positive cells increased significantly.Conclusion： The CD133＋ cells exist in U251 glioma cells,which have stem cell characteristics.
作者 杨伟现 欧阳琦 周月鹏 王存祖 张志坚 向定朝 许慧中 陆晓峰
机构地区 江苏大学附属医院神经外科 江苏大学基础医学与医学技术学院
出处 《江苏大学学报（医学版）》 CAS 2012年第1期27-30,共4页 Journal of Jiangsu University：Medicine Edition
基金 江苏省卫生厅面上科研项目资助(H2006040)
关键词 胶质瘤 CD133+细胞 肿瘤干细胞 glioma CD133＋ cells cancer stem cells
分类号 R739.4 [医药卫生—肿瘤]
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参考文献12

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同被引文献40

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引证文献1

江苏大学学报（医学版）
2012年 第1期

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