大黄酸对高脂血症小鼠肝脏脂肪含量的影响

Effect of rhein on the liver fat content of hyperlipidemia mouse model

目的:观察大黄酸对高脂诱导的高脂血症小鼠肝脏脂肪含量调节作用。方法:以高脂肪饮食诱导制作小鼠高脂血症模型,高脂血症模型随机分为大黄酸组(6只)、模型组(6只),同龄C57BL/6正常小鼠为对照组(6只)。治疗8周后,观察各组的体质量增长率,摄食量,肝脏脂肪含量,血清谷丙转氨酶(ALT),谷草转氨酶(AST)和血肌酐(SCr)。结果:模型组和大黄酸组的进食量较对照组低(P<0.01),而大黄酸组的进食量与模型组比较差异无统计学意义(P>0.05)。实验结束时,对照组、模型组和大黄酸组的体质量平均增长率分别为1.79%,10.69%,6.23%,大黄酸组体质量平均增长率较模型组明显降低(P<0.01)。对照组、模型组和大黄酸组的肝脏脂肪含量分别为2.71%,16.49%,8.36%,大黄酸组的肝脏脂肪含量较模型组明显降低(P<0.01)。3组治疗后的AST,ALT和SCr水平差异无统计学意义(P>0.05)。结论:大黄酸有抑制脂肪在肝脏沉积的作用,且不良反应小。

Objective： To observe the regulation of rhein on the hyperlipidemia mouse model induced by high fat diet.Methods： The hyperlipidemia mouse models induced by high fat diet were performed successfully and were randomly divided into rhein group（6 mice）,model group（6 mice） and control group（6 mice） were normal C57BL/6 mice.After treatment of 8 weeks,growth rate in body weight,food intake,liver fat content,serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and serum creatinine（SCr） were observed.Results： The food-intake in model group and rhein group decreased than those in control group（P〈0.01）,while there was no significant difference between model group and rhein group（P〉0.05）.At the end of the experiment,the average weight growth rate in control group was 1.79%,while it was 10.69% in model group and 6.23% in rhubarb group.The average weight growth rate in rhein group was significantly lower than that in model group（P〈0.01）.The liver fat content is 2.71% in control group,16.49% in model group,while it was 8.36% in rhubarb group;the liver fat content was significantly lower than that in model group（P〈0.01）.After treatment,the levels of AST,ALT,and SCr in three groups were no significant differences（P〉0.05）.Conclusion： Rhein have the function of inhibiting fat deposition in the liver,and the toxic side effects were very low.