三七总皂苷对大鼠脊髓损伤后GS的表达及运动功能恢复的影响

Effect of tPNS on GS expression and motor function recovery after spinal cord injury in rats

目的探讨三七总皂苷(total panax notoginseng saponins,TPNS)对大鼠脊髓半横断损伤后运动功能恢复的作用以及对谷氨酰胺合成酶(glutaminesynthetase,GS)表达的影响。方法将大鼠随机分为正常组、溶媒对照组和TPNS组,实验组大鼠建立脊髓半横断模型,TPNS组损伤处为脊髓右侧T10段。损伤后15 min,腹腔注射剂量为20 mg/kg的三七总皂苷,每天给药1次,溶媒对照组注射等量生理盐水。术后1、3、7、14、21、28 d进行行为学指标检测;采用免疫生物化学方法检测脊髓损伤远侧端GS表达的变化。结果行为学结果表明,该浓度的三七总皂苷能促进大鼠脊髓损伤后运动功能的恢复,其中损伤后7 d和14 d的BBB评分表明,三七总皂苷组大鼠运动功能恢复程度明显高于溶媒对照组。免疫组化结果表明脊髓半横断损伤后,在损伤脊髓远侧端GS的表达损伤侧强于对侧,损伤侧GS的表达趋势为1、3 d逐渐增强,7 d达高峰,在14 d时GS的表达逐渐下降,至28 d仍略高于正常组。三七总皂苷组和溶媒对照组相比,GS的表达在相同时间点优于对照组,尤其是3 d和7 d。结论三七总皂苷促进大鼠脊髓损伤后运动功能的恢复,这可能与其促进GS表达,从而改善脊髓再生的微环境有关。

Objective To investigate the effect of total panax notoginseng saponins（tPNS） on motor function recovery after semi-transverse spinal cord injury and its association with expression of glutaminesynthetase（GS）.Methods The rats were randomly divided into normal group,vehicle control group and tPNS group,the rats in experimental group were induced the spinal cord hemisection model,in TPNS group the spinal cord injury was at the right side of the T10th segment.After injury 15 min,dose of 20 mg/kg of PNS were injected into the abdominal cavity,once daily;in vehicle control group injected with normal saline.The target behaviors were detected at 1st,3rd,7th,14th,21st and 28th day after operaection;the GS expression changes of spinal cord injury in far side were detected with immunohistochemical detection.Results Behaviors showed that tPNS at the this concentration significantly promote the recoery of motor function,in which,at 7th and 14th day after injury,the BBB cores showed that the motor function recovery in tPNS group was significantly higher than that in vehicle control group;the immunohistochemistry results showed that the spinal cord hemisection injury in the distalspinal cord injury the expression of GS was stronger than the contralateral side of injury;the GS expression tends in the damage side were increased at 1st to 3rd day gradually,and reached the peak at 7th day,after 14th day,decreased gradually and to 28th day was still slightly higher than the normal group.Compared with vehicle control group,GS expression in PNS group at the same time stronger than that in control group,especially in the 3rd and 7th day.Conclusion Promoting recovery of motor function of spinal injury in rats,the machnism of tPNS may be associated with the increased GS expressive to improve the microenvironment for spinal cord regeneration.