动物体内海洛因代谢产物分析

Analysis of heroin metabolites in vivo

将吗啡、海洛因分别给予大鼠,建立成瘾动物模型,用气相色谱联用质谱(Gas chromatography-mass spectrometry)方法分析大鼠体毛、尿液中吗啡及6-单乙酰吗啡水平。结果显示:连续给药14天后检测分析,大鼠体毛中吗啡、6-单乙酰吗啡水平分别为2.64±0.9 ng·ng·mg^(-1).88±0.6ng·mg·mg^(-1),大鼠尿液中吗啡、6-单乙酰吗啡水平分别为27.7±0.6μg·mL^1、5.2±0.2μg·mL^1,与对照组比,有显著性差异(P<0.01)。海洛因在动物体内主要代谢产物为吗啡和6-单乙酰吗啡。

Rats were treated with morphine and heroin to build up an animal model of addiction. Levels of 6-monoacetylmorphine and morphine in body hair and urine of rat were determined with gas chromatography - mass spectrometry. The results showed that after a continuous treatment of 14 days the levels of morphine and 6-monoacetylmorphine in body hair were （2.64 ± 0.9） and （2.88 ±0.6） μg·mL^1, while those in urine were （27.7 ±0.6） and （5.2 ± 0.2）μg·mL^1, respectively, which were significantly different with the control group （P 〈0.01）. Morphine and 6-monoacetylmorphine were the major heroin metabolites in animals.