RP-HPLC法测定人血浆和尿液中美罗培南浓度

Determination of meropenem in humam plasma and urine by RP-HPLC

目的建立测定人血浆和尿液中美罗培南含量的RP-HPLC法。方法血浆样品经乙腈沉淀蛋白,二氯甲烷2次提取去杂质,取水相进样;尿液稀释后直接进样。以甲硝唑为内标,色谱柱为Capcell Pak MG C18(4.6 mm×150 mm,5μm)柱,流动相为乙腈-0.02 mol/L NaH2PO4溶液(体积比10∶90),流速为1.0 mL/min,检测波长为298 nm,柱温为30℃。结果美罗培南在血浆样品和尿样中的线性范围分别为0.2～100.0μg/mL和2～1 000μg/mL,提取回收率平均值分别为80.4%和101.1%,方法回收率平均值分别为99.2%和100.3%,日内、日间RSD值均低于8.8%。结论该方法简便、灵敏、专属性强,能满足人血浆和尿液中美罗培南含量测定及药动学研究的需要。

Objective To develop a simple and rapid HPLC method for the simultaneous determination of meropenem in human plasma and urine.Methods Plasma samples were deproteinized with acetonitrile followed by extraction with dichloromethane and injection of the upper aquous phase and urine samples were diluted with mobile phase.The analysis was conducted on a Capcell Pak MG C18 column.The flow rate was 1.0 mL/min and the column temperatunre was 30 ℃.The detecting wavelengh was at 298 nm.The mobile phase consisted of acetonitrile-0.02 mol/L biphosphate sodium（10∶90）.Results The linear range of meropenem in plasma and urine samples were 0.2-100.0 μg/mL and 2-1 000 μg/mL respectively.The average extraction recovery ratio in plasma and urine samples were 80.4% and 101.1%,and the average method recovery ratio in plasma and urine samples were 99.2% and 100.3%,respectively.The RSD of intra-and inter-day were both less than 8.8%.Conclusion The method is simple,rapid and accurate,which is suitable for the study of pharmacokinetics of meropenem.