HPLC法测定乌参醒脑滴丸中二苯乙烯苷的含量

Determination of stilbene glucoside in Wushen Xingnao drop pill by HPLC

目的建立高效液相色谱法测定乌参醒脑滴丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(二苯乙烯苷)含量的方法。方法采用SunFireTM C18(250 mm×4.6 mm,5.0μm)色谱柱,柱温为30℃;以乙腈-水(体积比20∶80)为流动相,流速为1.0 mL.min-1;检测波长为320 nm。结果二苯乙烯苷在12.75～85.0μg.mL-1范围内与峰面积呈良好的线性关系,平均回收率为99.8%,RSD为2.16%。结论该法可用于乌参醒脑滴丸中二苯乙烯苷的含量测定。

Objective To establish a method for the determination of stilbene glucoside in Wushen Xingnao（WSXN） drop pill with HPLC.Methods Chromatography was performed on a SunFireTMC18（250 mm ×4.6 mm,5.0 μm） at 30 ℃.The mobile phase was composed of acetonitrile-water（20∶80）with a flow rate of 1.0 mL·min-1.The detection wavelength was 320 nm.Results The calibration curve of stilbene glucoside was linear in the range of 12.75-85.0 μg·mL-1.The average recovery for stilbene glucoside was 99.8%（RSD=2.16%）.Conclusion The method can be used for quantitative determination of Wushen Xingnao drop pill.