荻植株再生体系建立

Establishment of plantlet regeneration system using seeds of Triarrherca sacchariflora(Maxin.)Nakai

以成熟种子为外植体建立了荻植株再生体系。荻成熟种子经20%"84"消毒液处理20 min,接种于MS+4%蔗糖(pH 5.5)培养基中培养5 d,萌发率为92%,褐化率为8%,污染率为0。萌发种子继代转入愈伤诱导培养基MS+1mg/l 6-BA+1mg/l 2,4-D+0.5mg/l NAA+4%蔗糖(pH 5.5),光照条件下愈伤组织诱导率为86%,黑暗条件下愈伤组织诱导率64%。荻愈伤组织在MS+1mg/L 6-BA+0.1mg/L2,4-D+0.5mg/L NAA+4%蔗糖(pH 5.5)进行不定芽诱导,不定芽发生率为91%。无根丛生芽继代转入不定根诱导培养1/2MS+0.25mg/L IBA+0.25mg/L NAA+4%蔗糖(pH 5.5),5天可见不定根形成,15天后不定根发生率为92%。研究还发现,荻种子萌发后可直接继代转入不定芽分化培养基形成健壮不定芽,不定芽诱导率为95%。

The plantlet regeneration system was established using mature seeds of Triarrherca sacchariflora （ Maxin. ） Nakai as explants. The MS with 4% sucrose（pH 5.5） was used as the germination medium of seeds treated with 20% thimemsal （＂84＂ disinfection solution） for 20 minutes, in which 92% of the seed germination rate ,8% of the mortality were obtained after 5 days culture. The germinated seeds were subcultured to the calli inducing medium MS ＋ lmg/L 6 - BA ＋ lmg/L 2,4 - D ＋ 0.Smg/L NAA ＋ 4% sucrose（pH 5.5）. The calli formation rate was more than 86% under illumination conditions, and that was 64% under dark conditions. The adventitious buds differentiation rate was 91% with the adventitious buds indu- ing medium MS ＋ I mg/L 6 - BA ＋ 0. I rag/L2,4 - D ＋ 0.5 mg/L NAA4 % sucrose （ pH 5.5）. The adventitous roots were induced with the adventitons roots inducing medium 1/2MS ＋ 0.25 mg/L IBA ＋ 0.25 mg/L NAA ＋ 4% sucrose（ pH 5.5） 5 days late, the rooting rate was 92% after 15 days culture. The adventitous buds were also induced after the germinated seeds were subculutred directly to the adventitous buds differentiation medium. The inducing rate of adventitous buds was 95 %.