青枯菌接种的2个花生抗感品种中几种酶活性的变化

The Study on Changes of Enzymes Activities in Resistant and Susceptible Cultivars of Peanut after Ralstonia solanacearum Inoculation

为给花生的抗病育种提供理论基础,用不同浓度的青枯菌接种2个抗感花生品种,分5个时期收获样品,用分光光度计法测量酶活性。感病品种的4种酶活性前期升高,而后期下降。而抗病品种的酶活性前期升高缓慢,后期升高速度越来越快。前期抗病品种酶活性不是都高于感病品种,后期抗病品种的酶活性则都高于感病品种。对照组2个品种花生的酶活性始终变化不大,它们之间酶活性也没有明显差异。PAL、CAT、PPO和POD作为植物的保护性酶类均参与了病程反应,其表达模式的差异可能与花生的青枯病抗性密切相关。

In order to provide theoretical guidance for resistant disease breeding of peanut,resistant and susceptible cultivars of peanut were vaccinated by Ralstonia solanacearum with different concentrations,the samples were collected in five periods,and enzymatic activities were measured by spectrophotometer method.The activities of 4 enzymes in susceptible cultivars increased in earlier stage,but declined in later stage.Enzymatic activities of resistant cultivars increased slowly in earlier stage,but increased more and more rapidly in later stage.Not all of the 4 enzymatic activities of resistant cultivars were higher than that of susceptible cultivars in earlier stage,but all of enzymatic activities of resistant cultivars were higher than that of susceptible cultivars in later stage.Changes of enzymatic activities in the control groups were not obvious,and the differences of enzymatic activities in two cultivars were not significant too.PAL,CAT,PPO and POD as protective enzymes participated in the course reaction,their expression pattern differences were closely related with Ralstonia solanacearum resistance of Ar achis hypogaea probablely.