环境中胆固醇降解菌的分离与鉴定

Isolation and Identification of Cholesterol-Degrading Strains in the Environment

本研究利用富集培养法从水塘底的污泥中分离出12株能以胆固醇为唯一碳源生长的菌株,并初步筛选出4株降解能力较强的菌株,这4个菌株在30℃、200r/min的培养条件下,96h内对30μg/mL的胆固醇的降解率分别为51.25%、36.65%、55.62%和29.59%。且它们适合生长的pH范围广,均在第12h后进入生长的稳定期。其中2个菌株(L4、L5)经形态特征以及16SrDNA序列分析,初步鉴定为肺炎克氏杆菌(Klebsiella pneumoniae)。

In this paper,12 strains that utilized cholesterol as sole carbon and energy sources were isolated from the sludge by the enrichment culture method.Four strains were selected for degradation of cholestero1.In the culture conditions of 30℃ and 200 r/min,the degradation rate to cholesterol of 4 strains were 51.25%,36.65%,55.62% and 29.59% respectively,with 30 μg/mL concentrations within 96 hours.The suitable pH values for their growth were wide,and the strains went into the stationary phase at the twelfth hour.L4 and L5 strains were identified to be Klebsiella pneumoniae by morphological characteristics and the similarity analysis of the 16S rDNA sequence.