空心莲子草不同部位水浸提液对蚕豆、玉米化感作用机制的研究

The Study on Allelopathy Mechanism of Aqueous Extracts from the Different Organizations of Alternanthera philoxeroides Griseb on Vicia faba and Zea mays

用空心莲子草为研究材料,以农作物蚕豆、玉米为受试植物,采用培养皿滤纸法测定空心莲子草对受试农作物种子萌发率的影响,并检测幼苗的生理指标丙二醛(MDA)含量、超氧化物歧酶(SOD)活性、过氧化物酶(POD)活力以及蚕豆根尖细胞微核率变化,来探讨化感作用机制。结果表明,空心莲子草根、茎、叶不同浓度水浸提液对受试农作物种子萌发率具有不同程度的抑制作用,且处理浓度越高抑制作用越强,水浸提液使受体幼苗体内的MDA含量增加,SOD活性、POD活性先增加后降低趋势,使蚕豆根尖细胞微核率上升。空心莲子草水溶性化感物质使受试农作物受到了氧化胁迫,抗氧化酶系统、蚕豆根尖细胞有丝分裂受到了抑制作用,从而影响了农作物幼苗的萌发和生长。

The study used Alternanthera philoxeroides as material,the subjects plants were Vicia faba and Zea may.The effects of Alternanthera philoxeroides Griseb on the seed germination of two species were studied by using culture dish filter paper method.The physiological index of tested crops MDA content,peroxidase （POD） activity changes,micronucleus rate changes of Vicia faba root-tip cell were detected.The result showed aqueous extracts form Alternanthera philoxeroides Griseb were affected to different degrees for the tested Vicia faba and Zea mays germination rate,made seedlings malondialdehyde （MDA） content to increase,made seedlings superoxide dismutase （SOD） and peroxidase （POD） activity firstly to increase and then to decrease trend,also made root tip cells of Vicia micronucleus rate to increase.The tested crops were oxidative stressed by water-soluble allelochemicals from Alternanthera philoxeroides Griseb.Antioxidant enzyme systems and root tip cells mitosis were inhibited.The aqueous extracts from the different organizations Alternanthera philoxeroides Griseb affected the germination and growth of Vicia faba and Zea mays seedlings.