重度子痫前期患者的体液因子与免疫细胞因子的相关性分析

Correlation analyses between humoral factors and cytokines in severe preeclampsia patients

目的探讨重度子痫前期患者血浆中体液因子内皮素(ET)、血管紧张素Ⅱ(AngⅡ)和免疫细胞因子白介素-2(IL-2)、白介素-10(IL-10)、γ-干扰素(γ-IFN)和转化生长因子-β1(TGF-β1)在重度子痫前期发病中的意义及其相关性分析。方法分别采用放射免疫法和双抗体夹心酶联免疫吸附测定法测定44例重度子痫前期孕妇(病例组),35例正常孕妇(对照组)妊娠晚期血浆中ET、AngⅡ和IL-2、IL-10、γ-IFN、TGF-β1水平。分析ET和AngⅡ与IL-2、IFN-γ、IL-10、TGF-β1、IL-2/IL-10比值、IFN-γ/IL-10比值等各细胞因子之间的相关性。结果①病例组的血浆IL-2水平和血浆TGF-β1水平高于对照组(P<0.001,P<0.01);病例组的血浆IL-10水平低于对照组为,P<0.001。②病例组血浆中IL-2/IL-10比值和IFN-γ/IL-10比值高于对照组(P<0.001和P<0.01)。③病例组血浆中ET水平为高于对照组P<0.01;病例组血浆中AngⅡ水平与对照组相比无显著差异P>0.05。④病例组血浆ET水平与IL-2水平、IL-2/IL-10比值、IFN-γ/IL-10比值的相关性有显著的统计学意义,病例组的血浆AngⅡ水平与血浆IL-2水平相关性有显著的统计学意义(P<0.01),病例组的血浆IL-10水平与血浆IFN-γ水平相关性有统计学意义(P<0.01)。结论免疫应答激活和免疫耐受不足共同参与了重度子痫前期的发生和发展,内皮细胞的损伤与免疫失衡相互协同促进了病程进展。

[ Objective ] To investigate the role of T-helper cell subtype including cytokines intedeukin-2 （IL-2）, interferon-γ （IFN-γ）, interleukin-10 （IL-10）, transforming growth factor-β1 （TGF-131） and humoral coordination factors angiotensin ]1 （Ang II ）, endothelin （ET） on the pathogensis of severe preeclampsia （sPE） and study the correlation of cytokines and humoral coordination factors. [ Methods ] 44 cases of sPE and 35 cases healthy pregnant women as control group were studied. Plasma cytokine levels including IL-2, IFN-γ, IL-10 and TGF-β1 were assessed by Enzyme linked immunosorbent assay （ELISA） and calculated Thl/Th2 （IL-2/IL-10 and IFN-γ/IL-10） ratios. Plasma levels of endothelin （ET） and angiotensin II （Ang II ） were detected by radioimmunoassay. [ Results ] ① Plasma levels of IL-2 and TGF-β1 in sPE group were higher than that in control group （P 〈0.001, P 〈0.01）; plasma levels of IL-10 in sPE group were lower than that in control group （P 〈0.001）. ② IL-2/IL-10 ratio and IFN-γ/IL- 10 ratio of plasma in sPE group were higher than that in control group （P 〈0.001 and P 〈0.01）. ③ Plasma levels of ET in sPE group were higher than that in control group, whereas Ang I] levels in two groups had no differences（P 〉 0.05）.④ Plasma ET ]evels in sPE group had correlation with plasma IL-2 levels, IL-2/IL-10 ratio and IFN-γ/IL-10 ratio. Plasma Ang II levels in sPE group had correlation with plasma IL-2 levels and Plasma IL-10 levels in sPE group had correlation with plasma IFN-γ levels. [ Conclusion ] Overly immune activation and immune intolerance participate in the pathogensis and development of sPE together; endothelial lesion in coordination with immune imbalance promote the progression of sPE.