治疗森林脑炎用人免疫血清制备

Investigation About Treatment of Tick-borne Encephalitis Employing Preparated Immune Serum

目的:监测森林脑炎病毒感染人体后不同时间血清抗森林脑炎抗体水平和抗森林脑炎抗体血清体外保存(-50℃)时间,进行治疗森林脑炎用人免疫血清的制备。方法:应用间接免疫荧光抗体技术检测血清抗森林脑炎病毒抗体(IgG)滴度,确定人体感染森脑病毒后产生高滴度抗体时间和血清抗体体外保存时间。结果:人体感染森脑病毒2～3个月时血清抗森林脑炎病毒抗体(IgG)滴度最高、感染6个月抗体水平开始下降;血清体外保存6个月抗体水平保持不变,9个月后开始下降,24个月均无抗体。血清抗体滴度越高体外保存时间越长。结论:可以采集森林脑炎病毒感染后2～3个月已知高滴度抗体人群的血液,制备成治疗用免疫血清,分装冰冻(-50℃)保存。在次年森脑高发期及时用于临床病人的治疗,这将提高治疗效果,对减少该病的死亡率和致残率具有重要意义。

Objective：To monitor of tick-borne encephalitis virus at different time after infecting with the human serum antibody level against tick-borne encephalitis and time that serum antibody against tick-borne encephalitis in vitro（-50 ℃）.Do research on treatment of tick-borne encephalitis employing preparated immune serum.Methods： To detect serum anti-borne encephalitis virus antibody（immunoglobulin G） titer with the technology of indirect immunofluorescence antibody.Make sure the time after being infected by tick-borne encephalitis virus time that producing high antibody titer and time to preserve high serum antibody in vitro.Results： 2-3 months After the human body infecting with the tick-borne encephalitis virus the serum antibody（immunoglobulin G） titer against tick-borne encephalitis is highest.After 6 months,antibody level begins to decline.But level remains unchanged in vitro.After 9 months,it starts to decline.After 24 months there is no antibodies.The higher serum antibody titer keeps the longer time it stays in vitro.Conclusion： To collect the blood that was infected with high titers of antibody for 2~3 months.,Prepare immune serum and pack,frozen（-50 ℃） and preserve.Apply to clinical treatment in the high incidence of Tick-borne encephalitis disease in the following year.This will enhance the treatment effect,it has meaning to reduce the mortality rate and disability rate.