EPO对新生大鼠缺氧缺血性脑损伤NSE表达的影响

Effect of erythropoietin on the expression of NSE in neonatal rats with hypoxic ischemic brain damage

目的:观察促红细胞生成素(EPO)对新生大鼠缺氧缺血性脑损伤后神经元特异性烯醇化酶表达的影响。方法:7日龄SD大鼠随机分成假手术对照组、EPO治疗组、缺氧缺血(HIBD)组。造模后在不同时间点检测脑质量损伤百分比、皮层脑组织HE染色、免疫组化观察皮层神经元特异性烯醇化酶(NSE)的表达。结果:与假手术对照组相比较,HIBD组大鼠脑组织病理变化明显,EPO治疗组能明显改善大鼠脑组织形态变化和脑质量损伤百分比(P<0.05);HIBD组大鼠造模48 h后可见部分NSE阳性表达出现在神经元外,造模后第4天NSE的阳性表达达到高峰;在相同时间点EPO治疗组可明显减少NSE阳性表达颗粒在神经元间隙的出现;HIBD组大鼠在造模后48h和8 d NSE阳性细胞数目均少于同日龄对照组和EPO组(P<0.05)。结论:EPO在HIBD发生早期起到神经保护的作用,减少神经元的坏死,减轻缺氧缺血导致的脑功能损伤。

Aim ： To observe the effect of erythropoietin on the expression of NSE （ neuron specific eno-lase） in neonatal rats with hypoxic ischemic brain damage. Methods： Sprague-Dawley rats （7-day old）were randomly divided into sham operation group, EPO treatment group and HIBD group. The histologi-cal （HE staining） and immunohistochemistry methods were used to determine the pathological changesand the NSE expression levels in the brain at different time points. Results： Compared to the sham oper-ation group, the rats of HIBD group showed significant pathological change, and the histological changesand the brain damage were improved significantly in EPO treatment group （ P 〈 0. 05 ） at every samplingpoint. 48 h after modeling , NSE expressions were observed in intercellular spaces in the HIBD group.After 96h, NSE expression increased sharply in intercellular spaces in the HIBD group. However, NSEexpressions were significantly reduced in the EPO treatment group at the same sampling point. The num-ber of NSE-positive cells in the HIBD group was smaller than that of the sham operation group and EPOgroup （P 〈 0. 05） after modeling 48 h and 8 d. Conclusion： In the early stage of HIBD, immediate ad-ministration of erythropoietin can play a neuroprotective role, reducing neuronal necrosis and improvingthe brain dysfunction.