Quality assessment of local Trichogramma species (Hymenoptera, Tri- chogrammatidae) to control the olive moth Prays oleae Bern. (Lepidop- tera, Yponomeutidae)

Assessment of quality of biological control agents is an important step for the success of any biocontrol program.Experiments were carried out to select candidate Trichogramma species for use in biological control of the olive moth Prays oleae Bern(Lepidoptera,Yponomeutidae).Thirteen local strains were preselected on the basis of the geographical origin and the availability from mass production of the rearing host Ephestia kuehniella ZELL.(Lepidoptera,Pyralidae) under laboratory conditions.Selection tests were focusing on acceptance and preference for P.oleae eggs.In the acceptance test,a single parasitoid female was offered P.oleae eggs on "tricho-card" for 6 hours at 25°C,70% HR and L16:D9 and assessed for parasitism after 6 days.The preference was determined by offering the choice and observing contacts with eggs of the target pest P.oleae and eggs of the host E.kuehniella.Parasitism was therefore assessed 6 days after the experiment under similar laboratory conditions.In host acceptance test,most eggs of P.oleae were parasited by strains belonging to the species T.oleae,T.bourarachae and T.cacoeciae.These strains also showed a high number of contacts with olive moth eggs in preference tests.However,a small number of T.bourarachae observed ovipositions resulted in successful parasitism.Therefore,T.oleae and T.cacoeciae were selected as candidates for further experiments.

Assessment of quality of biological control agents is an important step for the success of any biocontrol program. Experiments were carried out to select candidate Trichogramma species for use in bi- ological control of the olive moth Prays oleae Bern （ Lepidoptera, Yponomeutidae）. Thirteen local strains were pre - selected on the basis of the geographical origin and the availability from mass production of the rearing host Ephestia kuehniella ZELL. （ Lepidoptera, Pyralidae） under laboratory conditions. Selection tests were focusing on acceptance and preference for P. oleae eggs. In the acceptance test, a single para- sitoid female was offered P. oleae eggs on ＂tricho -card＂ for 6 hours at 25℃, 70% HR and L16：D9 and assessed for parasitism after 6 days. The preference was determined by offering the choice and observing contacts with eggs of the target pest P. oleae and eggs of the host E. kuehniella. Parasitism was therefore assessed 6 days after the experiment under similar laboratory conditions. In host acceptance test, most eggs of P. oleae were parasited by strains belonging to the species T. oleae, T. bourarachae and T. cacoeciae. These strains also showed a high number of contacts with olive moth eggs in preference tests. However, a small number of T. bourarachae observed ovipositions resulted in successful parasit- ism. Therefore, T. oleae and T. cacoeciae were selected as candidates for further experiments.