摘要
目的观察莫诺苷对二磷酸腺苷(ADP)诱导兔血小板聚集后Ca2+浓度的影响。方法利用钙离子荧光探针(Fura-2 AM)法,通过记录5 min之内Fura-2在激发波长340 nm和380 nm处的荧光强度比值,检测不同条件下ADP诱导血小板聚集后Ca2+的变化。结果与空白对照组相比,莫诺苷能显著抑制由ADP诱导兔血小板聚集后Ca2+的升高(P<0.001)。结论莫诺苷可能通过降低Ca2+的上升达到抗ADP诱导的兔血小板的聚集,从而改善体外血液流变学。
Objective To explore the effects of morroniside on Ca2＋ in the condition of platelet aggregation in rabbits induced by adenosine diphosphate（ADP）.Methods The mobilization of cytosolic-free calcium after platelet aggregation induced by ADP was detected by Ca2 ＋-sensitive fluorescent indicator,Fura-2 AM and time scan measurement.Results Compared with the controls,morroniside significantly inhibited the increase of Ca2＋ induced by ADP（P〈0.001）.Conclusion Morroniside acts as an effective platelet aggregative antagonist by inhibiting the increase of platelet Ca2＋.
出处
《中国康复理论与实践》
CSCD
北大核心
2012年第2期131-133,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
“重大新药创制”科技重大专项(2009ZX09103-366)
国家自然科学基金项目(30973893)
北京市自然科学基金项目(7102077)
北京市教委(PXM2011_014226_07_000071)
2011年北京市卫生系统高层次卫生技术人才
关键词
莫诺苷
血小板聚集
钙
兔
morroniside
platelet aggregation
calcium
rabbits
