血小板活化因子乙酰水解酶与过敏性紫癜肾脏损害的关联性

Correlation of Platelet Activating Factor Acetylhydrolase and Henoch-Schonlein Purpura Nephritis

目的探讨血小板活化因子乙酰水解酶(PAF-AH)与过敏性紫癜(HSP)肾脏损害的相关性。方法收集2010年10月-2011年4月在宁夏医科大学总医院住院的HSP患儿70例,并按有无肾脏损害将其分为非紫癜性肾炎(HSPN)组和HSPN组。另选体检健康的50例儿童为健康对照组。采用实时荧光定量PCR技术检测其PAF-AH mRNA表达,同时采用双抗体夹心酶联免疫吸附法检测其血清PAF-AH、血管内皮生长因子(VEGF)、热休克蛋白70(HSP70)水平变化。采用SPSS 13.0软件进行数据处理。结果1.HSPN组PAF-AH mRNA及蛋白表达、VEGF及HSP70表达均较非HSPN组及健康对照组增高,差异均有统计学意义(Pa<0.01);非HSPN组PAF-AH蛋白表达、VEGF及HSP70表达均较健康对照组增高,差异均有统计学意义(Pa<0.01)。2.HSPN组PAF-AH与VEGF蛋白表达及HSP70蛋白表达均呈正相关(Pa<0.01)。结论 PAF-AH参与了HSP的发病过程,并且PAF-AH水平与肾脏损害程度呈正相关。

Objective To explore the correlation of platelet activating factor acetylhydrolase（PAF-AH） and Henoch-Schonlein purpura nephritis（HSPN）. Methods Seventy children with Henoch-Schonlein purpura（HSP） who were admitted into the General Hospital of Ningxia Medical University from Oct.2010 to Apr.2011 were selected,and according to whether they had the kidney damage,they were divi-ded into non-HSPN group and HSPN group.Fifty healthy children were collected as healthy control group.And real-time fluorescent quantitive polymerase chain reaction was used to detect the expression of PAF-AH mRNA.Serum PAF-AH,vascular endothelial growth factor（VEGF）,and heat shock protein 70（HSP70） levels were detected by using enzyme-linked immunosorbent assay.SPSS 13.0 software was used to analyze the data. Results 1.The expressions of PAF-AH mRNA and protein,VEGF and HSP70 in HSPN group were higher than those in non-HSPN group and healthy control group,which were significantly positive（Pa0.01）.The expressions of PAF-AH protein,VEGF and HSP70 in non-HSPN group were higher than those in healthy control group,which were significantly positive（Pa0.01）.2.PAF-AH was positively correlated with VEGF and HSP70 in HSPN group（Pa0.01）. Conclusion PAF-AH is involved in the pathogenesis of HSP,which has relevance with kidney damage.