活性黑5高效脱色菌的固定化及其脱色特性研究

Immobilization and Decolorization Behavior of High-efficient Decolorization Strain for Reactive Black 5

采用海藻酸钙包埋法固定活性黑5高效脱色菌,通过正交试验确定固定化的最优条件,考察了pH、温度、初始染料浓度和重复利用次数等因素对固定化菌体脱色特性的影响,同时通过投加固定化颗粒处理模拟染料废水研究其生物强化作用。结果表明,最优固定化条件为海藻酸钠浓度3%,CaCl2浓度2%,菌体量与包埋剂量之比2:1;固定化颗粒对染料脱色的最适pH为8左右,最适温度为30℃,具有耐低温、染料浓度耐受极限高和可重复利用等特性,但在强碱性、高温和重复利用多次后,其机械强度降低;固定化菌体对以葡萄糖为外加碳源的染料废水的脱色效果较好,且浓度以1 g/L为宜,在厌氧污泥反应器中投加固定化颗粒对染料去除效率有所提高。

High efficient decolorization strain for Reactive Black 5 was immobilized using calcium alginate embedding method. The optimum condition of immobilization was determined by orthogonal experiment. Effects of influent pH, temperature, initial dye concentration and times of reuse on the behavior of decolorization of RB5 were investigated. The bioaugmentation of immobilized strain was investigated by treating simulated dye wastewater. Results showed that the optimum condition of immobilization was sodium alginate 3%, CaCl22% and the ratio of biomass to embedding agent 2 ： 1. The optimum pH and temperature of decolorization were 8 and 30 ℃ respectively. The immobilized pellets had the characteristics of low temperature resistance, high dye concentration tolerance and reuse, but its hardness decreased under the condition of strong base, high temperature and multi-using. The decolorization effect of dye wastewater by immobilized bacteria was better when the concentration of glucose as external carbon source was 1 g/L. The addition of immobilized pellets in anaerobic reactors promoted the removal rate of dyes