摘要
目的研究地顶胞霉菌丝体(AMM)对猪血清致大鼠免疫性肝纤维化的保护作用。方法采用猪血清腹腔注射造成大鼠免疫性肝纤维化模型,用试剂盒测定血清丙氨酸氨基转移酶(ALT)、血清天门冬氨酸氨基转移酶(AST)的活性和肝脏匀浆中超氧化物歧化酶(SOD)的活性、丙二醛(MDA)的含量;HE染色观察AMM对免疫性肝纤维化大鼠肝脏形态学影响。结果 AMM(350、700 mg.kg-1)不同程度增加大鼠的体重,降低大鼠的肝脾指数及血清中的ALT、AST的含量,减少肝组织匀浆中MDA的含量,升高肝组织中SOD的活性,明显减轻病理损伤。结论 AMM对猪血清所致大鼠免疫性肝纤维化有一定的保护作用。
Objective To investigate the protective effects of AMM on pig serum-induced liver fibrosis in rats.Methods We established a rat model of pig serum-induced liver fibrosis,used HE staining for pathological study of the liver tissues,and examined under a light microscope.Levels of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and activity of superoxide dismutase（SOD）,content of malondialdehyde（MDA） of hepatic tissues were measured by commercial kits.Results AMM（350,700 mg·kg-1） significantly increased the weights of rats,reduced liver and spleen indices and the content of ALT,AST in serum.Furthermore,it also improved liver total antioxidant capacity including SOD and significantly blocked the increase in MDA of hepatic tissues.It could also markedly decline pathological injury.Conclusion AMM has significant protective effects on pig serum-induced immune hepatic fibrosis in rats.
出处
《安徽医药》
CAS
2012年第2期152-154,共3页
Anhui Medical and Pharmaceutical Journal
基金
安徽省教育厅自然科学基金重点项目(No KJ2009A031和KJ2010A164)
安徽医科大学校级博士基金项目(NoXJ200821)
