摘要
目的:探讨癌干细胞标记物CD44与ESA蛋白在舌癌淋巴道转移模型中表达情况及其表达意义。方法:采用癌细胞足垫注射法建立淋巴道转移模型,4周后,取转移淋巴结进行原代细胞培养,从中分离、纯化舌癌细胞进行连续传代培养,建立舌癌永生化细胞系,命名为Tca8113-Ml。以舌癌永生化细胞系Tca8113-Ml为研究对象,采用足垫注射细胞的方法建立淋巴道转移模型。4周后,收集淋巴结,免疫组织化学染色检测癌干细胞标记物CD44与ESA蛋白的表达情况。结果:采用Tca8113-Ml建立了舌癌淋巴道转移模型,4周后收集淋巴结,检测癌干细胞标记物CD44与ESA蛋白在舌癌转移淋巴结组织中均呈阳性表达。结论:癌干细胞标记物在淋巴结转移性癌组织中呈阳性表达,表明淋巴结转移灶中有癌干细胞存在,提示癌转移的"种子"细胞极有可能是癌干细胞。
Objective:To explore the expressions and significance of CSCs associated markers CD44 and ESA in the metastatic tongue carcinoma lymph nodes of mice.Methods:Lymphatic metastatic animal model of tongue carcinoma through foot pad injection of cancer cells was established and metastatic lymph nodes were collected.Cell cultivating technique was employed to induce metastasis in lymph node and to obtain immortalized tongue carcinoma cell line from the metastatic cell.The immortalllized tongue carcinoma cell line cells were subsequently injected into nude mice via the foot pad.The cancer stem cell associated markers,such as CD44 and ESA,were detected by immunohistochemical staining from specimens of metastatic lymph node 4 weeks after injection.Results:In the lymphatic metastatic tongue carcinoma cells,staining of CD44 and ESA were positive.Conclusion:In the lymphatic metastatic tongue carcinoma cells,staining of CD44 and ESA of the concerned surface markers of cancer stem cells were positive,which shows that there may exist CSCs in the metastatic lymph nodes,and hints that the 'seeds' of cancer metastasis may be CSCs.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第2期122-124,131,共4页
Chinese Journal of Immunology
基金
省高等学校优秀青年人才基金项目(No.2011SQRL082)
院级课题项目(No.by1002)资助