自制冻伤软膏治疗兔冻伤的病理观察

Pathological observation of effect of homemade frostbite ointment on the frostbitten rabbits

目的:考察自制冻伤软膏对兔冻伤模型的疗效。方法:选择新西兰大白兔20只,随机分为对照组和治疗组,每组各10只。备皮,脱毛,经液氮致深Ⅱ度冻伤后进行治疗。在冻伤后6h,9、22、36d取皮,经40%甲醛固定,石蜡包埋,切片,HE染色,封片固定,观察分析病理切片。结果:对照组和治疗组兔的病理切片有明显差异,治疗组的冻伤皮肤结构恢复明显优于对照组。结论:自制冻伤软膏对治疗兔皮肤冻伤有一定的疗效。

Objective： To investigate the effect of frostbite ointment made by ourselves on frostbitten rabbit models.Methods： Twenty New Zealand rabbits were randomly divided into control group and treatment group,10 rabbits in each group.Grade Ⅱ deep frostbite were prepared by using the liquid nitrogen after hair removal.Skin samples were taken 6 h,9,22,36 d after frost injury,fixed by 40% formaldehyde water solution,embedded by paraffin and cut into slices.The slices were pathologically analyzed after conventional hematoxylin-eosin（HE） staining.Results： The pathological sections in treatment group were significantly different from those in control group.Frostbitten skin of rabbits in treatment group recovered sooner and better than those in control group.Conclusion： Frostbite ointment made by ourselves has therapeutic effects on frostbitten skin of rabbits.