裂褶菌产内切β-1,3-葡聚糖酶培养基组成的优化研究

Study on the optimization of medium composition of endo-β-1,3-glucanase producing by Schizophyllum commune Fr.

采用恒温摇床培养法培养裂褶菌,研究了裂褶菌产内切β-1,3-葡聚糖酶培养基组成中的碳源、氮源及各种无机盐的单因素参数,并采用四因素三水平正交实验对主要影响因素进行分析,从而确定最佳的产酶培养基配方为:葡萄糖2%,牛肉膏0.3%,NH4Cl0.1%,KH2PO40.05%,MgSO40.05%。

The constant shaking was used to culture Schizophyllum commune Fr.,and the different single-factor parameters of carbon,nitrogen and various salt forming the medium of endo-β-1,3-glucanase producing by Schizophyllum commune Fr.was studied.At the same time,the four factors and three level orthogonal experimental was used to analyze the main factor.Finally,determining the optimum medium composition：glucose 2%,beef extract 0.3%,NH4Cl 0.1%,KH2PO4 0.05%,MgSO4 0.05%.