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响应面优化酶法提取青钱柳黄酮的工艺 被引量:10

Optimization of total flavonoids extraction from Cyclocaraya paliurus by enzymatic hydrolysis using response surface methodology
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摘要 研究了纤维素酶解法对青钱柳总黄酮的提取,考察了酶解浓度、酶解提取温度、酶解提取时间等单因素对青钱柳总黄酮提取得率的影响。在单因素实验基础上通过响应面分析优选出最佳的提取条件为:酶解时间为32.28min、温度为41℃、加酶量为0.57%,缓冲液pH为6.37,在此条件下青钱柳总黄酮的提取得率为9.227%。 The cellulose enzymatic-assisted extraction of total flavonoids from Cyclocarya paliurus by using response surface method which was on the basis of simple factor experiment has been studied.The optimum enzymatic-aided extraction conditions were:extraction time was 32.28min,temperature at 41℃ and cellulose enzyme amount was 0.57%,pH of extracting media was 6.37.Under these conditions,9.227% yield of total flavonoids was obtained.
出处 《食品工业科技》 CAS CSCD 北大核心 2012年第5期250-253,共4页 Science and Technology of Food Industry
基金 广西科技厅科技攻关项目(桂科攻07180063)
关键词 青钱柳 总黄酮 酶法提取 提取得率 响应面法 Cyclocaraya paliurus total flavonoids enzymatic extraction extraction rate response surface method
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