摘要
研究微波对中性蛋白酶水解贻贝蛋白条件的影响,探讨了在一定的微波功率/时间,加酶量、酶解温度和时间对蛋白质回收率的影响。单因素实验先确定因素水平,再通过响应面确定最佳的工艺条件,酶解条件分别是时间为118.8min,加酶量为0.754%,微波功率为189.7W,温度分别为46.9℃,该条件下得到的最大蛋白质回收率为74.83%。
The impact of microwave extraction technique on neutrase hydrolyzing mussel was investigated.Under certain conditions of microwave power/radiation time,enzyme concentration,enzyme temperature and time influence recovery of hydrolysis has been discussed.The best extraction process conditions were determined by preferable level of factor which was decided by univariate test as well as response surface methodology.The results showed that the best conditions of enzyme hydolyzing mussel were：time was 118.8min,neutrase concentration was 0.754%,microwave power was 189.7W,temperature was 46.9℃,the hydrolization rate could reach 74.83%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第5期258-261,共4页
Science and Technology of Food Industry
基金
上海市教育委员会重点学科建设项目(J50704)
关键词
微波
酶法水解
贻贝
microwave
enzyme hydrolization
mussel
