摘要
目的探讨来氟米特(LEF)联合甲氨蝶呤(MTX)治疗大鼠胶原诱导的关节炎(CIA)的骨保护作用。方法建立CIA模型后,大鼠分为模型对照组、LEF组、MTX组、MTX+LEF(联合组);另设立空白对照组。用ELISA分析核因子κB受体活化因子的配体(RANKL)和白细胞介素17(IL-17)水平。结果与空白对照组比较,CIA大鼠模型外周血RANKL、IL-17水平明显升高(P<0.05)。与模型对照组比较,LEF组、MTX组、联合组大鼠模型外周血RANKL及联合组IL-17的水平均明显下降(P<0.05);与MTX组比较,联合组外周血RANKL、IL-17水平明显下降(P<0.05)。结论在成功建立的CIA大鼠模型中,LEF联合MTX对类风湿关节炎有协同骨保护作用,可能与下调外周血RANKL、IL-17水平有关。
Objective To evaluate the effects of combined use of leflunomide and methotrexate on receptor activator of nuclear factor-κB liqand（RANKL） and interleukin-17（IL-17） in collagen-induced arthritis（CIA） in rats.Methods The CIA rat models were established and divided into 4 groups of MC（model control）,L（leflunomide treated）,M（methotrexate treated） and LM（treated with leflunomide and methotrexate）.Serum RANKL and IL-17 were determined by ELISA.The results were compared to those in blank control group（BC）.Results Serum levels of RANKL and IL-17 were significantly higher in group MC than those in group BC（P0.05）.Compared with group MC,RANKL concentrations in groups of L,M and LM were obviously decreased（P0.05）.Compared to group M,serum levels of RANKL and IL-17 in group LM were notably down-regulated（P0.05）.Conclusion Combined use of leflunomide and methotrexate has a synergistic protection on CIA through down-regulating the serum level of RANKL and IL-17 in rats model.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第5期509-512,共4页
Jiangsu Medical Journal
基金
江苏省普通高校研究生科研创新计划项目(762)
南京市医学科技发展项目(YKKI1101)
