电刺激阴茎海绵体神经测定大鼠勃起功能的方法探讨

Measuring erectile function in rats through electrical stimulation of the cavernous nerve

目的:探讨电刺激阴茎海绵体神经(CN)测定大鼠勃起功能的方法。方法:选择4月龄雄性SD大鼠20只,体质量(425±25)g。左颈总动脉内插管,直接法持续监测大鼠平均动脉压(MAP);左侧海绵体内插管测定阴茎海绵体内压(ICP);前列腺右侧叶前外侧表面暴露右侧CN,通过电刺激CN诱发阴茎勃起。电刺激参数为:5V,2ms,25Hz,每次刺激持续1min,间隔5min重复电刺激,共刺激3次。电刺激CN前的(ICP/MAP)×100(rR)代表阴茎海绵体的静息状态;电刺激CN后的(ICP最大值/MAP)×100(bR)代表阴茎海绵体的勃起状态或大鼠的基础勃起功能;bR/rR代表阴茎勃起后ICP的增高程度。结果:电刺激CN使ICP明显升高,MAP变化不大,表明电刺激可有效诱发勃起。ICP、MAP、rR、bR、bR/rR等各项指标3次重复测定之间的差异均无统计学意义(均P>0.05),表明电刺激法结果稳定。rR、bR和bR/rR总的平均值分别为(12.00±2.62)%、(67.68±11.28)%和(5.85±1.80)。结论:电刺激阴茎CN测定大鼠勃起功能的方法稳定易重复,能够客观准确地评估阴茎的勃起程度,是研究阴茎勃起功能的重要技术,值得在国内推广。

Objective： To investigate the feasibility of measuring erectile function in rats through the electrical stimulation of the cavernous nerve （CN）. Methods： Twenty 4-month-old male SD rats （weight, 425 ± 25 g） were used in our study. The erectile response was expressed as a ratio of intracavernous pressure（ICP）and mean arterial pressure（MAP）, （ICP/MAP）× 100,during electrostimulation of the right CN. The electrical-stimulation parameters were 5 V, 2 ms, 25 Hz, and 1 rain for each stimulation and once again every 5 minutes. Before electrostimula- tion,the（ICP/MAP） ×100 （abbr, rR）represents resting state of penis. After electrostimulation, the（ICPmax/ MAP） × 100（abbr, bR）represents fundamentally erectile state of penis, bR/rR stands for the increased degree of ICP induced by electrostimulation. Results：Through the electrical stimulation of the CN, the ICP significantly increased and MAP remained stable, demonstrating reliability of the electrostimulation. The electrical-stimulation parameters, such as ICP, MAP, rR, bR, bR/rR,were not significantly different between three replicate measurements,demonstrating stability of the electrostimulation. The overall means of rR, bR and bR/rR were（12.00 ± 2. 62） %, （67.68 ±11.28） % and（5.85 ±1.80）, respectively. Conclusions：Measuring erectile function in rats through the electrostimulation of the CN is reliable and stable and can objectively reflect the erectile response in rats. Thus, it is an important technique to explore erectile function and deserves further study in China.