生物信息学法分析猪带绦虫苹果酸脱氢酶结构与功能

Bioinformatics Analysis on the Structure and Function of Malate Dehydrogenase Gene of Taenia solium

目的:分析和预测猪带绦虫苹果酸脱氢酶的结构和特性,用于指导其生物学功能的实验研究。方法:利用美国国家生物技术信息中心和瑞士生物信息学研究所的蛋白分析专家系统中有关基因和蛋白的序列和结构信息分析的工具,结合Pcgene和Vector NTI suite生物信息学分析软件包,从猪带绦虫全长cDNA质粒文库中识别苹果酸脱氢酶基因及其编码区,分析、预测该基因编码的蛋白质的理化特性、翻译后的修饰位点、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。结果:该基因编码332个氨基酸,为全长基因。Gen-Bank中与细粒棘球绦虫苹果酸脱氢酶序列同源性最高,理论分子量为36 459.2 Da。预测编码蛋白无跨膜区,无二硫键,稳定性较好。与吸虫属的苹果酸脱氢酶进化关系最近。结论:应用生物信息方法从猪带绦虫成虫cD-NA文库中筛选出了猪带绦虫核糖体cDNA全长序列并预测得到其结构与功能方面信息。

Objective： To analyze and predict the structure and characteristics of Taenia solium mal- ate dehydrogenase （MDH）, and so as to guide the experimental research on biological function of MDH. Methods： Tools about informatics analyis on sequences and structures of gene and protein in protein analysis expert system of bioinformatic institute of Switzerland, and those of state biological andtechnology information center of USA, combined with Pcgene and Vector NTI suite bioinformatics soft- ware pakege were employed to screen Taenia solium MDH gene and encoding region from cDNA plas- mid library to analyze and predict physicochemical properties of its encoding protein, modification site after translation, function domains, subcelluar location, topological structure, secondary structure,and 3D conformation and so on. Results： This gene encoded 332 amino acids, and was a full length gene. It was the most homologues to Taenia echinococcus MDH in GenBank. Its molecular weight in theory was 36 459. 2 Da. Its encoding protein was predicted to have no transmembrane region or disul-fide bonds, and was stable. Its evolutionary reltionship was closest to fasciola genus MDH. Conclu- sions： The cDNA sequence encoding MDH is screened from cDNA library of adult Taenia solium by bioinformatical method. The information about structure and function of Taenia solium MDH gene andprotein were obtained.